2018-01-03 16:51:252025-05-25 06:31:09
locus
BSU18450
BSU_18450
geneLength
4560
4563
outlinks
bsu
BSU18450
BSU_18450
Gene
Coordinates
2,010,070 → 2,014,632
2,010,070 2,014,632
The protein
Catalyzed reaction/ biological activity
2 L-glutamate NADP = L-glutamine 2-oxoglutarate NADPH (according to Swiss-Prot) 2 L-glutamate NADP( ) <=> L-glutamine 2-oxoglutarate NADPH
2 L-glutamate + NADP+ --> 2-oxoglutarate + H+ + L-glutamine + NADPH (according to UniProt)
The protein
Protein family
glutamate synthase family (according to Swiss-Prot) glutamate synthase family
glutamate synthase family (with [[protein|YerD]] and [[protein|GltB]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|YerD]]
[[this]]
The protein
[SW|Domains]
Glutamine amidotransferase type-2 domain (22-415)
Nucleotide binding domain (1060-1112)
[SW|Glutamine amidotransferase type-2 domain] (aa 22-415) (according to UniProt)
Nucleotide binding domain (1060-1112)
The protein
[SW|Cofactors]
[3 Fe- 4 S] cluster, FAD, FMN
Fe-S cluster [pubmed|29292548]
FAD (according to UniProt)
Biological materials
Mutant
GP807 (del ''[[gene|gltA]]-[[gene|gltB]]''::''tet'') , available in [SW|Jörg Stülke]'s lab
GP222 (''gltA'' under the control of p-xyl), available in [SW|Jörg Stülke]'s lab
1A808 ( ''gltA''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A808&Search=1A808 BGSC]
1A809 ( ''gltA''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A809&Search=1A809 BGSC]
BKE18450 (Δ[[gene|gltA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA
BKK18450 (Δ[[gene|gltA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA
BP123 (Δ[[gene|gltA]]-[[gene|gltB]]::''ermC'') , available in [SW|Fabian Commichau]'s lab
GP807 (Δ[[gene|gltA]]-[[gene|gltB]]::''tet'') , available in [SW|Jörg Stülke]'s lab
GP222 (''gltA'' under the control of p-xyl), available in [SW|Jörg Stülke]'s lab
1A808 ( ''gltA''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A808&Search=1A808 BGSC]
1A809 ( ''gltA''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A809&Search=1A809 BGSC]
BKE18450 ([[gene|gltA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA
BKK18450 ([[gene|gltA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA
Biological materials
lacZ fusion
pGP526 (in [[protein|pAC7]]), available in [SW|Jörg Stülke]'s lab [pubmed|14523131]
pGP919 (in [[protein|pAC5]]), available in [SW|Jörg Stülke]'s lab [pubmed|17183217]
pGP526 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab [pubmed|14523131]
pGP919 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab [pubmed|17183217]
Labs working on this gene/protein
[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
[SW|Jörg Stülke], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[SW|Fabian Commichau] University of Göttingen, Germany
[http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]
References
Original publications
labs
[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
[SW|Fabian Commichau] Brandenburg Technical University Cottbus-Senftenberg, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]